anti αv Search Results


αv integrin  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc αv integrin
αv Integrin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/Integrin+alphaV+Antibody/pmc04984616-120-17-24
Average 95 stars, based on 1 article reviews
αv integrin - by Bioz Stars, 2026-09
95/100 stars
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antibodies against integrin αvβ5  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology antibodies against integrin αvβ5
BC71 targets cell-surface GRP78 but not <t>αvβ5</t> <t>integrin</t> to induce apoptosis. (a) BC71 induces HUVECs apoptosis in a dose-dependent manner. HUVECs were treated with BC71 (concentration range: 12.5, 25, 50, 100 μM) for 24 h and apoptosis was determined using the cell death ELISA kit (Roche). (b) Anti-GRP78 N-terminal domain antibody blocked the apoptosis function of BC71 in a dose-dependent manner. The apoptosis of the combined treatment with increasing amount of anti-GRP78 N-terminal domain antibody and 100 μM BC71 for 24 h was measured using the Cell Death Detection ELISA. (c) Anti-GRP78 C-terminal domain antibody and (d) anti-αvβ5 antibody did not block BC71 induced apoptosis. For clarity of presentation, data were normalized with that of non-treated (VEGF only) cells, which was set as 1. Data are expressed as mean ± standard error of the mean. The results are representative of at least three independent experiments. Statistical significance was determined using ANOVA. *P < 0.05; **P < 0.01, n ≥ 3.
Antibodies Against Integrin αvβ5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/Integrin+%CE%B1V%2F%CE%B25+Antibody/pmc06085501-59-0-5
Average 93 stars, based on 1 article reviews
antibodies against integrin αvβ5 - by Bioz Stars, 2026-09
93/100 stars
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anti integrin αv  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti integrin αv
BC71 targets cell-surface GRP78 but not <t>αvβ5</t> <t>integrin</t> to induce apoptosis. (a) BC71 induces HUVECs apoptosis in a dose-dependent manner. HUVECs were treated with BC71 (concentration range: 12.5, 25, 50, 100 μM) for 24 h and apoptosis was determined using the cell death ELISA kit (Roche). (b) Anti-GRP78 N-terminal domain antibody blocked the apoptosis function of BC71 in a dose-dependent manner. The apoptosis of the combined treatment with increasing amount of anti-GRP78 N-terminal domain antibody and 100 μM BC71 for 24 h was measured using the Cell Death Detection ELISA. (c) Anti-GRP78 C-terminal domain antibody and (d) anti-αvβ5 antibody did not block BC71 induced apoptosis. For clarity of presentation, data were normalized with that of non-treated (VEGF only) cells, which was set as 1. Data are expressed as mean ± standard error of the mean. The results are representative of at least three independent experiments. Statistical significance was determined using ANOVA. *P < 0.05; **P < 0.01, n ≥ 3.
Anti Integrin αv, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/Integrin+%CE%B1V+Antibody/pmc12504495-41-21-43
Average 94 stars, based on 1 article reviews
anti integrin αv - by Bioz Stars, 2026-09
94/100 stars
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antibodies against αvβ3  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology antibodies against αvβ3
BC71 targets cell-surface GRP78 but not <t>αvβ5</t> <t>integrin</t> to induce apoptosis. (a) BC71 induces HUVECs apoptosis in a dose-dependent manner. HUVECs were treated with BC71 (concentration range: 12.5, 25, 50, 100 μM) for 24 h and apoptosis was determined using the cell death ELISA kit (Roche). (b) Anti-GRP78 N-terminal domain antibody blocked the apoptosis function of BC71 in a dose-dependent manner. The apoptosis of the combined treatment with increasing amount of anti-GRP78 N-terminal domain antibody and 100 μM BC71 for 24 h was measured using the Cell Death Detection ELISA. (c) Anti-GRP78 C-terminal domain antibody and (d) anti-αvβ5 antibody did not block BC71 induced apoptosis. For clarity of presentation, data were normalized with that of non-treated (VEGF only) cells, which was set as 1. Data are expressed as mean ± standard error of the mean. The results are representative of at least three independent experiments. Statistical significance was determined using ANOVA. *P < 0.05; **P < 0.01, n ≥ 3.
Antibodies Against αvβ3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/Integrin+%CE%B1V%2F%CE%B23+Antibody/pmc03073737-246-0-34
Average 93 stars, based on 1 article reviews
antibodies against αvβ3 - by Bioz Stars, 2026-09
93/100 stars
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rabbit polyclonal anti human integrin αv  (Biorbyt)
93
Biorbyt rabbit polyclonal anti human integrin αv
YAP1 regulates Wnt5A-induced <t>integrin</t> av and Smad2/3 activation. SKOV-3, OVCAR-3, and CAOV-4 cells were transfected with siRNA Scrambled (Scr) or Wnt5A. ( A ) Integrin <t>αv</t> expression level was assessed by immunoblotting in multicellular aggregates (MCAs) OvCa cells (upper panel) and quantifying bands from three experiments in the lower panel. ( B ) The cells were pre-treated with VP (5 μM) 1 h or treated with rhWnt5A (600 ng/mL) for 14 h alone or VP-pretreated + rhWnt5A then expression levels of integrin αv was determined in MCAs OvCa cells (Left panel), and quantification of bands (right panel). ( C ） The Wnt5A overexpressing OVCAR-3 (C3/OVCAR-3) and SKOV-3 clones (C9/SKOV-3) were treated with CWHM-12 (10 μM) for 24 h. Immunoblot of TGFβ1, pSmad2/3, YAP1 determined in MCAs OvCa cells (Left panel) and quantification of bands (right panels). The lower panel shows the quantification of bands from three independent experiments. GAPDH levels were used as an internal control, and results are expressed as mean ± SD. (D) The immunolocalization of pSmad2/3 and YAP-1 in CWHM-12-treated cells compared to control. Original magnification, ×400. *: p < 0.05, **: p < 0.01 and ***: p < 0.001 compared to untreated control cells (Ctrl) or Scr.
Rabbit Polyclonal Anti Human Integrin αv, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/Integrin+alphaV+antibody/pmc08773996-45-35-40
Average 93 stars, based on 1 article reviews
rabbit polyclonal anti human integrin αv - by Bioz Stars, 2026-09
93/100 stars
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mouse-anti-human αv  (Merck KGaA)
90
Merck KGaA mouse-anti-human αv
YAP1 regulates Wnt5A-induced <t>integrin</t> av and Smad2/3 activation. SKOV-3, OVCAR-3, and CAOV-4 cells were transfected with siRNA Scrambled (Scr) or Wnt5A. ( A ) Integrin <t>αv</t> expression level was assessed by immunoblotting in multicellular aggregates (MCAs) OvCa cells (upper panel) and quantifying bands from three experiments in the lower panel. ( B ) The cells were pre-treated with VP (5 μM) 1 h or treated with rhWnt5A (600 ng/mL) for 14 h alone or VP-pretreated + rhWnt5A then expression levels of integrin αv was determined in MCAs OvCa cells (Left panel), and quantification of bands (right panel). ( C ） The Wnt5A overexpressing OVCAR-3 (C3/OVCAR-3) and SKOV-3 clones (C9/SKOV-3) were treated with CWHM-12 (10 μM) for 24 h. Immunoblot of TGFβ1, pSmad2/3, YAP1 determined in MCAs OvCa cells (Left panel) and quantification of bands (right panels). The lower panel shows the quantification of bands from three independent experiments. GAPDH levels were used as an internal control, and results are expressed as mean ± SD. (D) The immunolocalization of pSmad2/3 and YAP-1 in CWHM-12-treated cells compared to control. Original magnification, ×400. *: p < 0.05, **: p < 0.01 and ***: p < 0.001 compared to untreated control cells (Ctrl) or Scr.
Mouse Anti Human αv, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/mouse+anti+human+%CE%B1v/pmc11874007-24-2-5
Average 90 stars, based on 1 article reviews
mouse-anti-human αv - by Bioz Stars, 2026-09
90/100 stars
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anti-av (cd51, clone amf7)  (Immunotec inc)
90
Immunotec inc anti-av (cd51, clone amf7)
YAP1 regulates Wnt5A-induced <t>integrin</t> av and Smad2/3 activation. SKOV-3, OVCAR-3, and CAOV-4 cells were transfected with siRNA Scrambled (Scr) or Wnt5A. ( A ) Integrin <t>αv</t> expression level was assessed by immunoblotting in multicellular aggregates (MCAs) OvCa cells (upper panel) and quantifying bands from three experiments in the lower panel. ( B ) The cells were pre-treated with VP (5 μM) 1 h or treated with rhWnt5A (600 ng/mL) for 14 h alone or VP-pretreated + rhWnt5A then expression levels of integrin αv was determined in MCAs OvCa cells (Left panel), and quantification of bands (right panel). ( C ） The Wnt5A overexpressing OVCAR-3 (C3/OVCAR-3) and SKOV-3 clones (C9/SKOV-3) were treated with CWHM-12 (10 μM) for 24 h. Immunoblot of TGFβ1, pSmad2/3, YAP1 determined in MCAs OvCa cells (Left panel) and quantification of bands (right panels). The lower panel shows the quantification of bands from three independent experiments. GAPDH levels were used as an internal control, and results are expressed as mean ± SD. (D) The immunolocalization of pSmad2/3 and YAP-1 in CWHM-12-treated cells compared to control. Original magnification, ×400. *: p < 0.05, **: p < 0.01 and ***: p < 0.001 compared to untreated control cells (Ctrl) or Scr.
Anti Av (Cd51, Clone Amf7), supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/anti+%CE%B1v++clone+69+6+5+/pm11886520-77-39-43
Average 90 stars, based on 1 article reviews
anti-av (cd51, clone amf7) - by Bioz Stars, 2026-09
90/100 stars
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pe-labeled anti-human αv-integrin antibody  (Cymbus Biotechnology)
90
Cymbus Biotechnology pe-labeled anti-human αv-integrin antibody
YAP1 regulates Wnt5A-induced <t>integrin</t> av and Smad2/3 activation. SKOV-3, OVCAR-3, and CAOV-4 cells were transfected with siRNA Scrambled (Scr) or Wnt5A. ( A ) Integrin <t>αv</t> expression level was assessed by immunoblotting in multicellular aggregates (MCAs) OvCa cells (upper panel) and quantifying bands from three experiments in the lower panel. ( B ) The cells were pre-treated with VP (5 μM) 1 h or treated with rhWnt5A (600 ng/mL) for 14 h alone or VP-pretreated + rhWnt5A then expression levels of integrin αv was determined in MCAs OvCa cells (Left panel), and quantification of bands (right panel). ( C ） The Wnt5A overexpressing OVCAR-3 (C3/OVCAR-3) and SKOV-3 clones (C9/SKOV-3) were treated with CWHM-12 (10 μM) for 24 h. Immunoblot of TGFβ1, pSmad2/3, YAP1 determined in MCAs OvCa cells (Left panel) and quantification of bands (right panels). The lower panel shows the quantification of bands from three independent experiments. GAPDH levels were used as an internal control, and results are expressed as mean ± SD. (D) The immunolocalization of pSmad2/3 and YAP-1 in CWHM-12-treated cells compared to control. Original magnification, ×400. *: p < 0.05, **: p < 0.01 and ***: p < 0.001 compared to untreated control cells (Ctrl) or Scr.
Pe Labeled Anti Human αv Integrin Antibody, supplied by Cymbus Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/pe+labeled+anti+human+%CE%B1v+integrin+antibody/us07238777-328-11-14
Average 90 stars, based on 1 article reviews
pe-labeled anti-human αv-integrin antibody - by Bioz Stars, 2026-09
90/100 stars
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anti-αv (cd51) antibody  (Enzo Biochem)
90
Enzo Biochem anti-αv (cd51) antibody
YAP1 regulates Wnt5A-induced <t>integrin</t> av and Smad2/3 activation. SKOV-3, OVCAR-3, and CAOV-4 cells were transfected with siRNA Scrambled (Scr) or Wnt5A. ( A ) Integrin <t>αv</t> expression level was assessed by immunoblotting in multicellular aggregates (MCAs) OvCa cells (upper panel) and quantifying bands from three experiments in the lower panel. ( B ) The cells were pre-treated with VP (5 μM) 1 h or treated with rhWnt5A (600 ng/mL) for 14 h alone or VP-pretreated + rhWnt5A then expression levels of integrin αv was determined in MCAs OvCa cells (Left panel), and quantification of bands (right panel). ( C ） The Wnt5A overexpressing OVCAR-3 (C3/OVCAR-3) and SKOV-3 clones (C9/SKOV-3) were treated with CWHM-12 (10 μM) for 24 h. Immunoblot of TGFβ1, pSmad2/3, YAP1 determined in MCAs OvCa cells (Left panel) and quantification of bands (right panels). The lower panel shows the quantification of bands from three independent experiments. GAPDH levels were used as an internal control, and results are expressed as mean ± SD. (D) The immunolocalization of pSmad2/3 and YAP-1 in CWHM-12-treated cells compared to control. Original magnification, ×400. *: p < 0.05, **: p < 0.01 and ***: p < 0.001 compared to untreated control cells (Ctrl) or Scr.
Anti αv (Cd51) Antibody, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/anti+%CE%B1v++cd51++antibody/pm37704610-311-1-5
Average 90 stars, based on 1 article reviews
anti-αv (cd51) antibody - by Bioz Stars, 2026-09
90/100 stars
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anti cd51 (integrin αv)  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH anti cd51 (integrin αv)
YAP1 regulates Wnt5A-induced <t>integrin</t> av and Smad2/3 activation. SKOV-3, OVCAR-3, and CAOV-4 cells were transfected with siRNA Scrambled (Scr) or Wnt5A. ( A ) Integrin <t>αv</t> expression level was assessed by immunoblotting in multicellular aggregates (MCAs) OvCa cells (upper panel) and quantifying bands from three experiments in the lower panel. ( B ) The cells were pre-treated with VP (5 μM) 1 h or treated with rhWnt5A (600 ng/mL) for 14 h alone or VP-pretreated + rhWnt5A then expression levels of integrin αv was determined in MCAs OvCa cells (Left panel), and quantification of bands (right panel). ( C ） The Wnt5A overexpressing OVCAR-3 (C3/OVCAR-3) and SKOV-3 clones (C9/SKOV-3) were treated with CWHM-12 (10 μM) for 24 h. Immunoblot of TGFβ1, pSmad2/3, YAP1 determined in MCAs OvCa cells (Left panel) and quantification of bands (right panels). The lower panel shows the quantification of bands from three independent experiments. GAPDH levels were used as an internal control, and results are expressed as mean ± SD. (D) The immunolocalization of pSmad2/3 and YAP-1 in CWHM-12-treated cells compared to control. Original magnification, ×400. *: p < 0.05, **: p < 0.01 and ***: p < 0.001 compared to untreated control cells (Ctrl) or Scr.
Anti Cd51 (Integrin αv), supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/anti+cd51++integrin+%CE%B1v+/pmc05469790-253-18-25
Average 90 stars, based on 1 article reviews
anti cd51 (integrin αv) - by Bioz Stars, 2026-09
90/100 stars
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anti-rat αv antibody  (Becton Dickinson)
90
Becton Dickinson anti-rat αv antibody
YAP1 regulates Wnt5A-induced <t>integrin</t> av and Smad2/3 activation. SKOV-3, OVCAR-3, and CAOV-4 cells were transfected with siRNA Scrambled (Scr) or Wnt5A. ( A ) Integrin <t>αv</t> expression level was assessed by immunoblotting in multicellular aggregates (MCAs) OvCa cells (upper panel) and quantifying bands from three experiments in the lower panel. ( B ) The cells were pre-treated with VP (5 μM) 1 h or treated with rhWnt5A (600 ng/mL) for 14 h alone or VP-pretreated + rhWnt5A then expression levels of integrin αv was determined in MCAs OvCa cells (Left panel), and quantification of bands (right panel). ( C ） The Wnt5A overexpressing OVCAR-3 (C3/OVCAR-3) and SKOV-3 clones (C9/SKOV-3) were treated with CWHM-12 (10 μM) for 24 h. Immunoblot of TGFβ1, pSmad2/3, YAP1 determined in MCAs OvCa cells (Left panel) and quantification of bands (right panels). The lower panel shows the quantification of bands from three independent experiments. GAPDH levels were used as an internal control, and results are expressed as mean ± SD. (D) The immunolocalization of pSmad2/3 and YAP-1 in CWHM-12-treated cells compared to control. Original magnification, ×400. *: p < 0.05, **: p < 0.01 and ***: p < 0.001 compared to untreated control cells (Ctrl) or Scr.
Anti Rat αv Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/anti+rat+%CE%B1v+antibody/us08445006-166-41-53
Average 90 stars, based on 1 article reviews
anti-rat αv antibody - by Bioz Stars, 2026-09
90/100 stars
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anti-αv integrin antibody l230  (Enzo Biochem)
90
Enzo Biochem anti-αv integrin antibody l230
YAP1 regulates Wnt5A-induced <t>integrin</t> av and Smad2/3 activation. SKOV-3, OVCAR-3, and CAOV-4 cells were transfected with siRNA Scrambled (Scr) or Wnt5A. ( A ) Integrin <t>αv</t> expression level was assessed by immunoblotting in multicellular aggregates (MCAs) OvCa cells (upper panel) and quantifying bands from three experiments in the lower panel. ( B ) The cells were pre-treated with VP (5 μM) 1 h or treated with rhWnt5A (600 ng/mL) for 14 h alone or VP-pretreated + rhWnt5A then expression levels of integrin αv was determined in MCAs OvCa cells (Left panel), and quantification of bands (right panel). ( C ） The Wnt5A overexpressing OVCAR-3 (C3/OVCAR-3) and SKOV-3 clones (C9/SKOV-3) were treated with CWHM-12 (10 μM) for 24 h. Immunoblot of TGFβ1, pSmad2/3, YAP1 determined in MCAs OvCa cells (Left panel) and quantification of bands (right panels). The lower panel shows the quantification of bands from three independent experiments. GAPDH levels were used as an internal control, and results are expressed as mean ± SD. (D) The immunolocalization of pSmad2/3 and YAP-1 in CWHM-12-treated cells compared to control. Original magnification, ×400. *: p < 0.05, **: p < 0.01 and ***: p < 0.001 compared to untreated control cells (Ctrl) or Scr.
Anti αv Integrin Antibody L230, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B1v/anti+%CE%B1v+integrin+antibody+l230/pmc02862273-151-57-61
Average 90 stars, based on 1 article reviews
anti-αv integrin antibody l230 - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


BC71 targets cell-surface GRP78 but not αvβ5 integrin to induce apoptosis. (a) BC71 induces HUVECs apoptosis in a dose-dependent manner. HUVECs were treated with BC71 (concentration range: 12.5, 25, 50, 100 μM) for 24 h and apoptosis was determined using the cell death ELISA kit (Roche). (b) Anti-GRP78 N-terminal domain antibody blocked the apoptosis function of BC71 in a dose-dependent manner. The apoptosis of the combined treatment with increasing amount of anti-GRP78 N-terminal domain antibody and 100 μM BC71 for 24 h was measured using the Cell Death Detection ELISA. (c) Anti-GRP78 C-terminal domain antibody and (d) anti-αvβ5 antibody did not block BC71 induced apoptosis. For clarity of presentation, data were normalized with that of non-treated (VEGF only) cells, which was set as 1. Data are expressed as mean ± standard error of the mean. The results are representative of at least three independent experiments. Statistical significance was determined using ANOVA. *P < 0.05; **P < 0.01, n ≥ 3.

Journal: EBioMedicine

Article Title: Proapoptotic Cyclic Peptide BC71 Targets Cell-Surface GRP78 and Functions as an Anticancer Therapeutic in Mice

doi: 10.1016/j.ebiom.2018.06.004

Figure Lengend Snippet: BC71 targets cell-surface GRP78 but not αvβ5 integrin to induce apoptosis. (a) BC71 induces HUVECs apoptosis in a dose-dependent manner. HUVECs were treated with BC71 (concentration range: 12.5, 25, 50, 100 μM) for 24 h and apoptosis was determined using the cell death ELISA kit (Roche). (b) Anti-GRP78 N-terminal domain antibody blocked the apoptosis function of BC71 in a dose-dependent manner. The apoptosis of the combined treatment with increasing amount of anti-GRP78 N-terminal domain antibody and 100 μM BC71 for 24 h was measured using the Cell Death Detection ELISA. (c) Anti-GRP78 C-terminal domain antibody and (d) anti-αvβ5 antibody did not block BC71 induced apoptosis. For clarity of presentation, data were normalized with that of non-treated (VEGF only) cells, which was set as 1. Data are expressed as mean ± standard error of the mean. The results are representative of at least three independent experiments. Statistical significance was determined using ANOVA. *P < 0.05; **P < 0.01, n ≥ 3.

Article Snippet: Antibodies against integrin αvβ5 (P1F76; Santa Cruz Biotechnology, Santa Cruz, CA, USA), GRP78 (A-10, Santa Cruz Biotechnology) and GRP78 (C-20, Santa Cruz Biotechnology) were used for neutralization.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Blocking Assay

YAP1 regulates Wnt5A-induced integrin av and Smad2/3 activation. SKOV-3, OVCAR-3, and CAOV-4 cells were transfected with siRNA Scrambled (Scr) or Wnt5A. ( A ) Integrin αv expression level was assessed by immunoblotting in multicellular aggregates (MCAs) OvCa cells (upper panel) and quantifying bands from three experiments in the lower panel. ( B ) The cells were pre-treated with VP (5 μM) 1 h or treated with rhWnt5A (600 ng/mL) for 14 h alone or VP-pretreated + rhWnt5A then expression levels of integrin αv was determined in MCAs OvCa cells (Left panel), and quantification of bands (right panel). ( C ） The Wnt5A overexpressing OVCAR-3 (C3/OVCAR-3) and SKOV-3 clones (C9/SKOV-3) were treated with CWHM-12 (10 μM) for 24 h. Immunoblot of TGFβ1, pSmad2/3, YAP1 determined in MCAs OvCa cells (Left panel) and quantification of bands (right panels). The lower panel shows the quantification of bands from three independent experiments. GAPDH levels were used as an internal control, and results are expressed as mean ± SD. (D) The immunolocalization of pSmad2/3 and YAP-1 in CWHM-12-treated cells compared to control. Original magnification, ×400. *: p < 0.05, **: p < 0.01 and ***: p < 0.001 compared to untreated control cells (Ctrl) or Scr.

Journal: Cells

Article Title: Wnt5A and TGFβ1 Converges through YAP1 Activity and Integrin Alpha v Up-Regulation Promoting Epithelial to Mesenchymal Transition in Ovarian Cancer Cells and Mesothelial Cell Activation

doi: 10.3390/cells11020237

Figure Lengend Snippet: YAP1 regulates Wnt5A-induced integrin av and Smad2/3 activation. SKOV-3, OVCAR-3, and CAOV-4 cells were transfected with siRNA Scrambled (Scr) or Wnt5A. ( A ) Integrin αv expression level was assessed by immunoblotting in multicellular aggregates (MCAs) OvCa cells (upper panel) and quantifying bands from three experiments in the lower panel. ( B ) The cells were pre-treated with VP (5 μM) 1 h or treated with rhWnt5A (600 ng/mL) for 14 h alone or VP-pretreated + rhWnt5A then expression levels of integrin αv was determined in MCAs OvCa cells (Left panel), and quantification of bands (right panel). ( C ） The Wnt5A overexpressing OVCAR-3 (C3/OVCAR-3) and SKOV-3 clones (C9/SKOV-3) were treated with CWHM-12 (10 μM) for 24 h. Immunoblot of TGFβ1, pSmad2/3, YAP1 determined in MCAs OvCa cells (Left panel) and quantification of bands (right panels). The lower panel shows the quantification of bands from three independent experiments. GAPDH levels were used as an internal control, and results are expressed as mean ± SD. (D) The immunolocalization of pSmad2/3 and YAP-1 in CWHM-12-treated cells compared to control. Original magnification, ×400. *: p < 0.05, **: p < 0.01 and ***: p < 0.001 compared to untreated control cells (Ctrl) or Scr.

Article Snippet: The following antibodies were used in this study: mouse monoclonal anti-human pSmad2/3 and mouse monoclonal anti-human Smad2/3 were purchased from (Santa Cruz Biotechnology Inc. Heidelberg, Germany), rabbit polyclonal anti-human TGFβ1, rabbit polyclonal anti-human YAP1, and rabbit polyclonal anti-human integrin αv (Biorbyt Ltd., Cambridge, UK); mouse monoclonal anti-human Wnt5A and rabbit polyclonal anti-human GAPDH rabbit polyclonal anti-human α-SMA (Abcam, Boston, MA, USA).

Techniques: Activation Assay, Transfection, Expressing, Western Blot, Clone Assay, Control

Model of Wnt5A involvement in EMT and mesothelial activation and clearance. Wnt5A derived from ovarian cancer cells through cytoskeletal rearrangement could directly or indirectly cause YAP1 phosphorylation, which translocates into the nucleus and induces TGFβ1, integrin αv, Wnt5A, and other EMT markers in this drawing. Integrin αv, in turn, may activate extracellular latent-TGFβ1 and play a pivotal role in the EMT process. In addition, YAP1 may cause retention of Smad2/3 in the nucleus, thereby prolonging their biological activity.

Journal: Cells

Article Title: Wnt5A and TGFβ1 Converges through YAP1 Activity and Integrin Alpha v Up-Regulation Promoting Epithelial to Mesenchymal Transition in Ovarian Cancer Cells and Mesothelial Cell Activation

doi: 10.3390/cells11020237

Figure Lengend Snippet: Model of Wnt5A involvement in EMT and mesothelial activation and clearance. Wnt5A derived from ovarian cancer cells through cytoskeletal rearrangement could directly or indirectly cause YAP1 phosphorylation, which translocates into the nucleus and induces TGFβ1, integrin αv, Wnt5A, and other EMT markers in this drawing. Integrin αv, in turn, may activate extracellular latent-TGFβ1 and play a pivotal role in the EMT process. In addition, YAP1 may cause retention of Smad2/3 in the nucleus, thereby prolonging their biological activity.

Article Snippet: The following antibodies were used in this study: mouse monoclonal anti-human pSmad2/3 and mouse monoclonal anti-human Smad2/3 were purchased from (Santa Cruz Biotechnology Inc. Heidelberg, Germany), rabbit polyclonal anti-human TGFβ1, rabbit polyclonal anti-human YAP1, and rabbit polyclonal anti-human integrin αv (Biorbyt Ltd., Cambridge, UK); mouse monoclonal anti-human Wnt5A and rabbit polyclonal anti-human GAPDH rabbit polyclonal anti-human α-SMA (Abcam, Boston, MA, USA).

Techniques: Activation Assay, Derivative Assay, Phospho-proteomics, Activity Assay